Im curious as to whether the software for LCMS (Labsolutions from Shimadzu) already has a quantification feature or do I have to compute and compare the AUCs to the standards manually? If so, what are the steps in the computation/quantification of compounds?
Coupled to a properly developed LC-MS method (the most important part), you will have to use known standards and use calibration tables for EACH compound to make any types of measurements. Contact a professional at your school for additional help.
Things to consider: quantifying requires a quantification method, where you assign retention time, m/z (for MS), Channel (for UV) etc. to your analytes/compounds, so they got recognized by the software. In this case, you will get peak areas (or area ratio in case of using an internal standard), and this can be called qualitative analysis. For quantitative analysis, you need to add the concentration of each calibration curve standard in order to quantify.
You can follow LabSolution training or watch tutorial videos on how to quantify using LabSolutions software.
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