What are the applications of proteomics in fish processing?

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When can E.coli secrete most of the extracelluar protein?

Hello, I'm doing optimization of an extracellular protein cloned in the pET26b vector along with PelB leader sequence. I want to know the best time at which cells can secrete most of the protein...

09 October 2019 6,989 7 View

How to optimize production of enzymes using E. coli BL21(DE3)?

Hi, Previously i have used shake flask method to optimize production of pectinase and then i have done its stability analysis. But now i have to increase its production using fermenter tank and...

04 May 2019 4,032 1 View

How to derivate compounds containing amino groups in aqueous solution??

I want to use gas chromatography to analyze substances containing amino group, but because of the strong polarity of amino group, it may damage the chromatographic column, so i need to derivate it...

02 March 2019 3,864 6 View

How to confidently predict the 3D structure of a protein?

I have tried to predicted the 3D structure by using multiple online softwares like I-TASSAR, MUSTER, Swiss-Model and (PS)2 but predicted 3D structure from each software has bad quality and can not...

10 November 2018 5,447 6 View

How to do molecular dynamics simulations for investigating the effect of non-synonymous mutations on protein structure through Discovery Studio??

Hello, I want to do simulations analysis for investigating the effect of non-synonymous mutations on protein structure through Discovery Studio. Kindly suggest me a protocol. Thanks

01 February 2018 2,558 4 View

Can anyone send me Setup of Simplot?

I need it for recombination analysis but i'm unable to download it on window 8.1.

07 August 2017 8,439 0 View

How can i get licence of discovery studio visualizer?

i want to get information about interactions between protein and ligand after docking and want to use discovery studio visualizer for it. I have tried to get its licence after registration but...

10 November 2016 9,292 13 View

What can be basic steps for the population/molecular or genetic analysis of a disease?

I want to do Population/ Molecular / Genetic analysis of a viral disease in my local population. So, for this purpose, can anyone help me to select appropriate methodology like what can be...

09 October 2016 2,781 0 View

How to create PDBQT file using autodock tools?

I am using autodock tools to create pdbqt file for autodock vina but when i try to save file it warns me that receptor protein contains no non-bonded atoms. Can anybody help me to solve this...

06 July 2016 7,011 8 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Image Processing and classification using keras/Tensorflow?

Do you have some recommendations approaches on Image processing and classification of plant diseases using TensorFlow/Keras?

02 March 2021 1,493 4 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View