What are the applications of proteomics in fish processing?

More Sana Tehreem's questions See All

When can E.coli secrete most of the extracelluar protein?

Hello, I'm doing optimization of an extracellular protein cloned in the pET26b vector along with PelB leader sequence. I want to know the best time at which cells can secrete most of the protein...

09 October 2019 7,192 7 View

How to optimize production of enzymes using E. coli BL21(DE3)?

Hi, Previously i have used shake flask method to optimize production of pectinase and then i have done its stability analysis. But now i have to increase its production using fermenter tank and...

04 May 2019 4,197 1 View

How to derivate compounds containing amino groups in aqueous solution??

I want to use gas chromatography to analyze substances containing amino group, but because of the strong polarity of amino group, it may damage the chromatographic column, so i need to derivate it...

02 March 2019 4,031 6 View

How to confidently predict the 3D structure of a protein?

I have tried to predicted the 3D structure by using multiple online softwares like I-TASSAR, MUSTER, Swiss-Model and (PS)2 but predicted 3D structure from each software has bad quality and can not...

10 November 2018 5,657 6 View

How to do molecular dynamics simulations for investigating the effect of non-synonymous mutations on protein structure through Discovery Studio??

Hello, I want to do simulations analysis for investigating the effect of non-synonymous mutations on protein structure through Discovery Studio. Kindly suggest me a protocol. Thanks

01 February 2018 2,725 4 View

Can anyone send me Setup of Simplot?

I need it for recombination analysis but i'm unable to download it on window 8.1.

07 August 2017 8,617 0 View

How can i get licence of discovery studio visualizer?

i want to get information about interactions between protein and ligand after docking and want to use discovery studio visualizer for it. I have tried to get its licence after registration but...

10 November 2016 11,118 13 View

What can be basic steps for the population/molecular or genetic analysis of a disease?

I want to do Population/ Molecular / Genetic analysis of a viral disease in my local population. So, for this purpose, can anyone help me to select appropriate methodology like what can be...

09 October 2016 2,954 0 View

How to create PDBQT file using autodock tools?

I am using autodock tools to create pdbqt file for autodock vina but when i try to save file it warns me that receptor protein contains no non-bonded atoms. Can anybody help me to solve this...

06 July 2016 8,598 8 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...

05 August 2024 853 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Simulation of metal drawing by Abaqus with UMAT?

Hello, colleagues. Recently, I have been working on a metal processing simulation with my UMAT in Abaqus. I have outlined the corresponding simulation, but I keep encountering issues that cause...

30 July 2024 7,062 1 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

What is the function of fusion tag GAMGSGIQRPTST?

I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?

23 July 2024 1,353 4 View