Sana Tehreem

10 Questions 10 Answers 0 Followers

Questions related from Sana Tehreem

When can E.coli secrete most of the extracelluar protein?

Hello, I'm doing optimization of an extracellular protein cloned in the pET26b vector along with PelB leader sequence. I want to know the best time at which cells can secrete most of the protein...

10 October 2019 7,131 7 View

How to optimize production of enzymes using E. coli BL21(DE3)?

Hi, Previously i have used shake flask method to optimize production of pectinase and then i have done its stability analysis. But now i have to increase its production using fermenter tank and...

05 May 2019 4,137 1 View

How to derivate compounds containing amino groups in aqueous solution??

I want to use gas chromatography to analyze substances containing amino group, but because of the strong polarity of amino group, it may damage the chromatographic column, so i need to derivate it...

03 March 2019 3,985 6 View

How to confidently predict the 3D structure of a protein?

I have tried to predicted the 3D structure by using multiple online softwares like I-TASSAR, MUSTER, Swiss-Model and (PS)2 but predicted 3D structure from each software has bad quality and can not...

11 November 2018 5,580 6 View

How to do molecular dynamics simulations for investigating the effect of non-synonymous mutations on protein structure through Discovery Studio??

Hello, I want to do simulations analysis for investigating the effect of non-synonymous mutations on protein structure through Discovery Studio. Kindly suggest me a protocol. Thanks

02 February 2018 2,678 4 View

Can anyone send me Setup of Simplot?

I need it for recombination analysis but i'm unable to download it on window 8.1.

08 August 2017 8,560 0 View

How can i get licence of discovery studio visualizer?

i want to get information about interactions between protein and ligand after docking and want to use discovery studio visualizer for it. I have tried to get its licence after registration but...

11 November 2016 10,963 13 View

What can be basic steps for the population/molecular or genetic analysis of a disease?

I want to do Population/ Molecular / Genetic analysis of a viral disease in my local population. So, for this purpose, can anyone help me to select appropriate methodology like what can be...

10 October 2016 2,895 0 View

What are the applications of proteomics in fish processing?

Hello, I want to get information about applications of proteomics in fish processing.

09 September 2016 612 2 View

How to create PDBQT file using autodock tools?

I am using autodock tools to create pdbqt file for autodock vina but when i try to save file it warns me that receptor protein contains no non-bonded atoms. Can anybody help me to solve this...

07 July 2016 8,287 8 View