What could be the other optional dyes that can work in bacterial cell staining that can work in tandem with propidium iodide for staining of bacterial cells.
I am currently using the Fpocket webserver which provides information about the present pockets in the protein structure, but currently having trouble in selecting individual pockets. Is there any...
21 January 2024 4,370 0 View
I am currently looking for any tools to analyze the probable docking sites intead of going for blind docking. Is there any bio-informatics tools or webserver that can help me further analyze the...
21 January 2024 1,036 4 View
Hi everyone I am currently looking for a structural database for nanoparticle / nanomaterial (s). Can someone provide any suggestions. Thanks in Advance.
01 January 2024 7,343 4 View
I want to investigate the structutre of my nanoparticles through RPBS web portal used for structural bioinformatics and drug designing. The lack of tutorials is making it really taking, any leads...
06 December 2023 839 2 View
I am performing compression molding of PCL to enhance its mechanical strength. The processed PCL obtained was then palletized and blended with the fillers to make a composite. My question is, will...
12 June 2023 4,276 0 View
What metrics do we use to assess ocean acidification? What is the potential impact of ocean acidification on the marine food chain?
04 May 2023 5,772 0 View
Consider a substance is used at with an concentration x, which is having absorbance greater than 4, how to troubleshoot the problem where x is a critical concentration.
16 February 2023 9,197 4 View
I have the EEG DEAP dataset in .dat format , by the process I can see the complete data of each candidates . but I want to store those data in a CSV file . can you please help me regarding this ?
12 July 2022 5,450 8 View
I want to calculate Cellular proliferation along with the population doubling time of MSCs cultured onto scaffolds for bone tissue regeneration. Can anyone suggest to me the required procedures...
25 March 2021 9,633 5 View
I am basically working on electrical charactering of supercapacitor. As the output of the supercapacitor is not stable, i needed the dc dc converter. and for there search article i need to check...
27 December 2020 8,586 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View