What could be the other optional dyes that can work in bacterial cell staining that can work in tandem with propidium iodide for staining of bacterial cells.
I want to use ANN-GA hybrid method for optimization. I know the use of ANN toolbox in matlab. but dont know where i can find the modelling equation of ANN so that i can use that for GA in matlab...
17 June 2020 6,518 5 View
25 July 2017 8,002 5 View
I collected benthic samples from 12 stations (3 samples per station across 4 locations) across mudflats from one estuary, in autumn & the following spring, totalling 72 samples. In addition, I...
10 July 2017 9,464 2 View
I want to build a low cost (not very accurate but with allowable margin of errors) solar irradiance sensor for my weather station using a photo transistor, but I am looking for the method of...
31 May 2017 530 3 View
What would you recommend for a simulation software to build a water based solar photovoltaic cooling system model with all major components specified with certain dimensions and capacities? The...
20 May 2017 5,643 2 View
With weather parameters like solar irradiance, ambient temperature, relative humidity in air, wind speed and its direction, how could they be measured accurately and reliably in periodic intervals...
14 May 2017 9,377 4 View
02 March 2021 3,060 3 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
28 February 2021 9,936 2 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View