As part of clinical chemistry automation, endpoint, and kinetic methods are used. What are the advantages and disadvantages of both ways, and which is preferred during analyte testing?
Good day, Ms. Cañeda!
End Point Method and Kinetic Method are two of the three (along with the Fixed Type method) Clinical Chemistry methods that are used for the estimation of various analytes in the laboratory. According to Madhu (2018), the main distinction between the two methodologies is that the Kinetic method measures the difference in absorbance between various sites as the reaction progresses, while the Endpoint method measures the total number of analytes involved in the reaction. That being said, the major advantages and disadvantages of these two approaches will most likely relate to the turnaround time for laboratory results, difference in precision of reaction rates, the cost-effectiveness of the reagents that will be employed, and the skills of the medical technologist in performing the tests.
According to a journal article by Srisawasdi et. al. (2007), two methodologies have been used in performing a Serum Cholesterol Determination Test and the result states that the kinetic approach required a lesser time for analysis and displayed less interference than the end point method. However, the end point method’s advantage over the kinetic method includes an improved accuracy as the latter’s imprecision fell within the criteria range of
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