Looking to purchase an imaging system. Anyone care to comment on the above systems?
Thanks
Hello We're currently attempting to create a plasmid stock of lentivirus. We're using a pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRE (#85453 addgene) in which we've removed the tdTomato and inserted a...
14 December 2023 3,842 1 View
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24 September 2023 7,180 6 View
Trying to PEGylate gold nanoparticles with HS-PEG5000-NHS
01 September 2022 5,939 3 View
I am trying to determine what statistical test I should use. I am comparing the results of a control group after treatment to the rest of the population. in this case it is teat scores from one...
03 July 2022 7,278 3 View
Hi all, I have been interested in validating our RNAseq data and have noticed some discrepancies with qPCR or droplet digital PCR for mature (exonic-exonic based probes). Does DESeq distinguish...
13 October 2021 4,458 2 View
So I was running an IR on a sample that I had thought (or rather hoped) was phenol (hydroxy benzene), but what came out was either trash and noise, or I simply did not have phenol. My professor...
04 October 2021 6,391 3 View
I have a question with regard to SMAP processing. I have been evaluating the Level 2 product (SMAP-Sentinel-1 L2 Radar/Radiometer 30-Second Scene 3km EASE-Grid Soil Moisture V003) and I was...
21 June 2021 4,244 2 View
Hello! I am hoping someone can provide some insight. We are trying to perform a no choice leaf disk assay using strawberry and F. occidentalis ( western flower thrips) females. We have starved...
12 June 2021 9,351 3 View
For my college assignment I need to produce a leaflet detailing the information required when assessing a dog for a diagnosis for hydrotherapy and physiotherapy and the impact this has on the...
11 May 2021 9,837 2 View
Hi! Does any have a suggestion for the best (quickest?!) way to find out if a particular mRNA sequence (from a paper) aligns to an exon or exon-exon junction of a particular gene? Thank you.
28 December 2020 550 3 View
I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...
01 August 2024 9,227 1 View
We are looking for projects for a team of professional developers About us We are a team of experienced developers with over 10 years of experience in various programming fields. Our mission is to...
30 July 2024 8,377 0 View
I'm using a InVia Renishaw Raman microscope system (785nm wavelength, 600l/mm grating density) to acquire Raman images on human brain samples, at 50um resolution. The samples are placed on MgF2...
30 July 2024 8,771 3 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,761 5 View
I am reaching out to seek your valuable advice and recommendations regarding the best software tools to use for this research. Specifically, I am looking for software with a user-friendly...
22 July 2024 3,794 1 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
22 July 2024 155 4 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View
What type of experimental design do l use and what statistical tools do l use for the hypothesis. What is the position of control group in the analysis?. Thanks
18 July 2024 3,581 3 View
I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...
17 July 2024 6,213 4 View
Image Processing Algorithms, Quantum Computing.
17 July 2024 7,958 2 View