Is there a way to batch-convert mass spectrometry cdf files to mzML files?

I have a set of roughly one hundred cdf files from a mass spectrometry experiment. I would like to analyse them using MetaboAnalystR, which requires mzML files as input. I know I can convert each...

28 March 2020 1,794 6 View

What is the best methodology for working with temporal data holes and deep neural networking?

Any data science and/or remote sensing experts have any insight pertaining to multivariate regression and deep neural networking with large feature set inputs and variable temporal data holes...

03 February 2020 5,246 2 View

Why are surrogates only added at the time of extraction, not at the time of sample collection and processing?

I am working on a study on pharmaceutical fate and uptake in plants. We are wondering about analyte degradation during the initial transport, processing, and storage of the soil and plant samples,...

30 October 2019 3,540 1 View

Is there a method or reference that shows how (or what is) the oxygen transmission rate (OTR) of water?

I have found multiple references on solubility however I can find nothing on transmissibility. The later is usually reserved for polymer films but in theory it should be determinable for water as...

05 August 2019 4,776 3 View

How is depth information encoded in the frequency of spectral interferograms obtained by Spectral domain optical coherence tomography?

I am trying to understand how the depth information in SD-OCT A-scans is obtained over distances far in excess of the source coherence length. From what I have gathered so far, it seems that the...

10 June 2019 6,783 2 View

How can I improve laser penetration on a confocal microscope in aortic tissue?

I am currently doing an experiment to image thick (~100 micron) cut pieces of aorta and am having a few problems. To imagine the shape of my tissue, I am cutting rings or bands of aorta and...

01 August 2018 920 3 View

Can BLAST perfrom a search including IUPAC ambiguity codes?

23 June 2017 8,927 3 View

What is the expected number of connected components in an Erdos-Renyi graph?

Let G(N,p) be an Erdos-Renyi graph, where N is the number of vertices, and p is the probability that two distinct vertices form an edge. If I plot 1-b0/N over log(p), then I obtain a curve which...

23 November 2016 6,443 3 View

Database of reagent off-target effects?

I remember seeing a recent website/database that listed commonly used cell culture and research chemical agents and their known off-target effects. Can anyone help me re-discover the site?

17 April 2016 5,311 2 View

How to conduct measured path analysis using Lisrel?

I am trying to understand how to use Lisrel to produce the best model for my survey data. I have read through the guides provided by the website but still do not seem to understand the...

19 February 2015 3,277 5 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Image Processing and classification using keras/Tensorflow?

Do you have some recommendations approaches on Image processing and classification of plant diseases using TensorFlow/Keras?

02 March 2021 1,493 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View