What are technics and marquer genes to identify Rhizobium strains?

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Ahmed Mansour Alzohairy

I think sequencing 16srRNA and ITS region will be appropriate for your purposes. I hope this answer may help you

Alex Ignatov

Ahmed,

16S can be used to group the strains into species, but for intraspecies diversity MLST has been developed. See :

1: van Berkum P, Badri Y, Elia P, Aouani ME, Eardly BD. Chromosomal and symbiotic

relationships of rhizobia nodulating Medicago truncatula and M. laciniata. Appl

Environ Microbiol. 2007 Dec;73(23):7597-604. Epub 2007 Oct 5. PubMed PMID:

17921275; PubMed Central PMCID: PMC2168047.

2: van Berkum P, Elia P, Eardly BD. Multilocus sequence typing as an approach for

population analysis of Medicago-nodulating rhizobia. J Bacteriol. 2006

Aug;188(15):5570-7. PubMed PMID: 16855247; PubMed Central PMCID: PMC1540022.

3: van Berkum P, Elia P, Eardly BD. Application of multilocus sequence typing to

study the genetic structure of megaplasmids in medicago-nodulating rhizobia. Appl

Environ Microbiol. 2010 Jun;76(12):3967-77. doi: 10.1128/AEM.00251-10. Epub 2010

Apr 23. PubMed PMID: 20418433; PubMed Central PMCID: PMC2893487.

If you work with narrow clonal group of strains (like population dinamics in one location) you can use any DNA fingerprinting method.