What are suitable culture medias for PBMC sub-populations after FACS sorting?

31 May 2014 0 2K Report

I would like to FACS sort (after labeling the PBMC sample with antibody cocktails) the PBMC cell types (like CD4+T cells, CD8+ T cells, B cells, DCs, NK cells etc) and culture them for my further experiments. Can anyone suggest respective culture medias for the cell types given above? If you are doing the similar kind of work or know any useful literature I can follow, please let me know. Any suggestion welcome.

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