The gene of interest that was knockdown in my cell is Myogenic differentiation factor 1 (MyoD) and I am using C2C12 wild-type cells. I was wondering if creating a knockout of MyoD would be more effective than having a knockdown cell line.
Both have advantages and disadvantages depending upon the gene in question and the question one is attempting to address. In your case, if the "knockdown" was >70% and if the "knockdown" is tolerated by cells, you should be able to move forward with your experiments. If not, you may need an inducible knockdown system that is not leaky.
Hello
May help you these links
Good Luck
http://nebula.wsimg.com/32579a4066f44f4276d761f8b7e99725?AccessKeyId=B88088AB6A6BB4657046&disposition=0&alloworigin=1
http://www.ncbi.nlm.nih.gov/pubmed/23211595
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