How to make an apparatus for testing membrane permeability and selectivity?

Good afternoon everyone! I just can't find drawings or a description of the creation of an apparatus for determining the permeability and selectivity of membranes in relation to gases and gas...

14 January 2021 9,205 4 View

Can anyone help to get ansys-fluent tutorial zip files ?

I need the following tutorial zip files to learn the basics of the software: Using sliding and dynamic meshes Modeling solidification Using the Eulerian Granular Multiphase Model with heat...

03 January 2021 5,571 5 View

Can I make mixture of cinnamic acid with morin flavanoid ?

I am working on a project for assessing the Antibacterial activity of various chemicals, I was wondering Can i make solution of those components using water as a solvent, depending on the fact...

19 November 2020 7,857 4 View

Fixation of whole rats organs in buffered formalin?

Dear colleagues, I would like to ask you if anyone has experience with the fixation of whole rat organs (heart, lungs, liver, kidneys, brain, testes) in 10% buffered formalin. Thank you, Roman

28 October 2020 3,175 3 View

How can you do biotynilation of antibodies in presence of gentamicin?

For some time we have used biotinylated antibodies for ELISA. Usually I use biotin N-hydroxysuccinimide ester to link biotin to antibodies (and I have used different carbodiimides for other...

28 July 2020 8,806 3 View

If you had to reference a publication which proves or at least rigorously argues that AI control problem is (un)solvable, what would you cite?

Links to such articles with some explanations would be great!

27 July 2020 7,035 6 View

What is the estimated projected price of electricity (or hydrogen) for bio-solar cells?

Hello, everyone! There are many reports about bio-based solar cells and their prospects in the scientific literature. All authors write that these devices will be low-cost. Does anyone know any...

13 July 2020 655 3 View

How to keep in memory the global ecological pause during the COVID-19?

Dear Researchers, I would like to raise a debate with you, regarding the impact of COVID-19 in the environment and climate change. We are a group of researchers that have launched a map, which...

08 June 2020 9,286 3 View

Can excess chlorination of drinking water have long term harmful effects on our health??

can excess chlorination of drinking water have long term harmful effects on our health: digestive problems...

19 May 2020 4,930 11 View

What statistical technique shall I follow?

Dear researchers, I want to compare three different means of the same group. For example, I want to know which school subject my students (independent variable)are good at depending on their...

02 May 2020 724 23 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View