I am new to the world of protein expression and purification and have no prior knowledge or experience about this topic. I will be starting to express my first 2 proteins in a couple of weeks. It will be the gpW and engrailed protein to be expressed using E. Coli. system. Before I start, I would like to know what background knowledge I would need before getting started (I have majored in chemistry with an emphasis on physical chemistry). Can you point out some resources from where I can build my knowledge, and get tips on the procedure and troubleshooting?
There are some resources or blogs, like that by addgene where you can learn the basics of protein expression. You may not understand all the terms used in these blogs but will learn while expressing the protein in the lab.
https://blog.addgene.org/plasmids-101-protein-expression
For basic molecular biology, see resources from Khan academy
https://www.khanacademy.org/science/biology/x324d1dcc:metabolism
I think the pET System Manual is a great resource. You should be able to just do a google search and find it to download.
Hi Catherine, QIAGEN has a handbook for high-level expression and purification of 6xHis-tagged proteins. It helped me a lot when I was a master's student. It's available for free downloading at the following link
https://www.qiagen.com/us/resources/resourcedetail?id=79ca2f7d-42fe-4d62-8676-4cfa948c9435&lang=en
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