Hi,Robert. I remember that 20-30% of the approved therapeutic proteins are currently being produced using E. coli as a host. Here is the ref you can use to answer your question, enjoy reading

Hey,

Escherichia coli is a suitable host for the milligram to gram-scale production of natural products and secondary metabolites with interesting bioactivities (antibiotic, antibacterial, anticancer, etc.). Often these compounds accumulate in the membrane and have to be extracted using organic solvents.

Reviews & Example studies:

Article Systems metabolic engineering of Escherichia coli for the he...

Article Systems metabolic engineering of Escherichia coli for produc...

Article Systems Metabolic Engineering of Escherichia Coli for Gram S...

Article Metabolic engineering of microorganisms for production of ar...

Article Microbial Platform for Terpenoid Production: Escherichia col...

Article Metabolic engineering of Escherichia coli for the production...

Best,

Michael

E. coli most studied microorganism in term of producer of bioactive compaunds.

Currently, there are many biopharmaceuticals (recombinant proteins) that are expressed in E.coli, especially, as inclusion bodies which are subsequently extracted, purified and conditioned for sale to the public.

Among the most recognized are:

-Filgrastim

-Molgramostim

-Human insulin

-Interferon alfa 2b

-Interferon Beta 1b

-Interferon Beta 1a

-Interferon

-Somatropin

Article Recombinant protein expression in Escherichia coli: Advances...

Hi

Approximately, around 30% of biopharmaceutical recombinant proteins is currently produced by E. coli. Recombinant protein in E. coli can produced in either intra-, periplasmic, or extracellularly expressed protein, with the majority of efforts defaulting to intracellular production in the form of inclusion bodies such as Interferons and Streptokinase , etc. For more information please see the attached article.

Lu Chen much appreciated the article was very informative.

hello,

Bacteria such as Bacillus cereus produces more bio-pharmaceutical enzymes.

for example: fibrinolytic protease which can degrade the fibrin clot and helps in fighting against cardio vascular diseases. And these intracellular proteases can be recovered easily by disrupting cell membrane using anionic or cationic surfactants. Ex : SDS, CTAB.

thanks to everyone for helping