How does the N value be counted for gut content analysis?

I read some articles on mollusc and fish diet analysis. The formula for percentage frequency of occurrence stated above: %F0 = Nei/Ne x 100 and percentage numerical abundance : %NI : Ni/N x...

15 October 2020 4,568 3 View

Why is it necessary to vortex and incubate in dark the sample and DPPH solution and other DPPH questions?

Hi all, In the determination of Antioxidant capacity of fruit samples, I used DPPH assay and have a few questions regarding the DPPH assay 1) why is it necessary to vortex and incubate in dark...

30 August 2020 246 3 View

How to renew the Scopus index status of a journal?

Dear Researchers, Our journal (ISSN no. 2070-4658) was part of the Scopus index till 2017. Now, it is not in the Scopus index as the last two years no issue has been published. However, the...

24 August 2020 7,203 3 View

Hello,i extract my plants using rotary evaporator and i got crude extract.How should dilute it?i have no idea on how to dilute it?Can someone helpme?

dilute it in ethanol or dmso? which solution is better as i used 95% of ethanol as my extraction solvent?

23 March 2020 5,971 6 View

Can I set "20 uL reaction volume" in PCR cycler machine, while my actual sample is only 10 or 15 uL?

This is a stupid but quite technical question. We have a PCR machine that has 20 uL minimum reaction, so it can't be set into 15 or 10 uL in the machine. Is this going to affect the result...

22 March 2020 3,497 4 View

Is there any SOP to measure pharmaceutical clean room and if none can we develop our own method to measure the room size?

06 March 2020 866 1 View

How to test degree of polymerisation of aged kraft paper impregnated in oil?

kraft paper was impregnated in mineral oil at different temperatures and duration. my project is to get the DP value at different duration. in order to be measured by viscometer, the sample must...

20 January 2020 2,234 3 View

What is the equation to explain how one possible organic side product is formed in the synthesis of 1-bromobutane?

And show the chemical properties window of this side product. Explain how this product can be separated from the desired product. I have already identified that the possible organic side products...

29 December 2019 6,170 4 View

Validation for Intracerebroventricular Microinjection of Streptozotocin in Rats?

Hi, I'm planning to induce sporadic Alzheimer's disease (AD) in male Sprague Dawley rats (3 months old, 270-350g) through bilateral intracerebroventricular microinjection of 3 mg/kg streptozotocin...

25 November 2019 9,723 3 View

What is the meaning of negative activation energy?

I am prepared (Al, Co) co-doped TiO2 thin films by spray pyrolysis technique. The electrical measurement was performed by the four-probe point method, in the range of temperature 3ok (Room...

22 October 2019 526 4 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

In frequency domain alpha band(8-16hz), beta band(16-32hz), gamma band(32-64hz), sub-band(64-128hz) are same as LL,LH,HL,HH in wavelet decomposition?

I am working on single trial motor imagery data i.e. EEG data. I am following a paper which has a dataset from BCI challenge IV (dataset 2b). After removing noise and extracting all the data from...

23 February 2021 5,503 1 View

MVN, how to conciliate contradictory results from Multivariate normality and Multivariate outliers test?

Dear colleagues, I'm working with Path analyses in lavaan and MVN packages. There are some results that for me are confusing and features from the MVN that I do not know how to set. My dataset is...

18 February 2021 190 3 View

Is it possible to obtain a negative cronbach alpha?

Good evening everyone. If a questionnaire has 4 factors measuring different constructs as for example CAMI questionnaire measuring attitudes toward the mentally ill that has four factors:...

18 February 2021 3,223 3 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View

Can we have constructs of Cronbach alpha value with more than 0.9 in social science research? What is recommended in this problem?

My study consists of a conceptual framework with seven constructs measured in seven point Likert scale. According to pilot study, only three constructs have Cronbach alpha value more than 0.9...

12 February 2021 9,368 2 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

Fractional derivative users, are you on the same page with me on the validity of the fractional order parameter, say alpha?

Taking a fractional ODE problem as an example. It has come to my attention that if the fractional order parameter is in the interval (0,1], many only presents results for alpha=0.9, 0.99. I saw...

08 February 2021 9,671 3 View

How can I select the best plasmid after sequencing?

I have transformed a drought-related gene of 1.7kb into pGEMt vector (E.coli DH5 alpha strain). After sequencing the plasmids (3 replicates) 4 changed amino acids are visible at different...

06 February 2021 336 3 View