No title

23 November 2020 7,077 3 View

Do we need Quantum Mathematics?

18 October 2020 830 2 View

Whether gap junction will effect neuronal capacitance measurement ?

i use whole cell patch to measure neuronal tau and resistant. with the equation tau=RC, Capacitance is calculated. I wonder if neuron has coupled with other neurons via gapjunction, the measured...

24 February 2019 7,702 7 View

Are there any online courses about adult education?

I am researching the topic on teaching foreign language to older adults, are there any online courses about adult education ?

05 July 2018 4,030 12 View

How to dilute solid milk from stomach?

We are trying to detect oxytocin from milk in the rat stomach. We found out that milk there are solid like beancurd. any suggested method to dilute them before centrifuge? Many thanks for help!

23 April 2018 777 1 View

What is the difference between headstage and bipolar electrode on making field stimulation?

for example, I am going to give a field stimulation (square-wave pulses of 100 mV 1000Hz duration 1ms). and I have a axon patch 700B with two headstage and one pencilshapged concentric bipolar...

19 February 2018 670 1 View

Is there anything wrong with limit theory in present infinite set theory?

We understand from the infinite related mathematics history that in present classical mathematics (such as classical mathematical analysis and classical set theory) basing on classical infinite...

17 February 2018 3,688 2 View

No title

10 January 2018 8,153 7 View

Is the barber paradox really a problem for present set theory-------what is the basic theory for one-to-one coresponding operation?

20 December 2017 2,594 4 View

How a point mutation in DNA open reading frame alters gene expression level?

The point mutation in ORF increases the protein expression level. What is regulatory element involved in this function? Thanks!

23 May 2017 1,917 3 View

Plasmid amplification in S2 cell?

When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...

09 February 2021 5,423 1 View

Apart from MOFA what other software is available for analysis of a multi-omic study?

Study bringing together genomics, proteomics etc together

02 February 2021 2,856 2 View

How can i improve my DNA purity for whole genome sequence that has high 260/230 ratio ?

I am performing whole genome sequence for gram negative acinetobacter baumannii using two platforms illumina miswq and oxford nanopore. Thus i require a very high yield and high purity. I am using...

31 January 2021 355 2 View

Is there any alternative to BUSCO for searching orthologues within genomes available?

I have a need to search for single-copy conservative orthologs within several plant genomes and used BUSCO tool for that. However, I found that the results produced by different versions on the...

29 January 2021 7,764 6 View

How to annotate list of gene names using Bash/R?

I have a list of gene names from deep sequencing from a constructed CRISPR knockout library. I was hoping to write a script to annotate all the gene names with a quick description of product...

27 January 2021 8,035 1 View

Question regarding investigation of the nature of bacteria: whether it can cause human diseases or not?

Hi all, Considering that one has the whole genome sequence (annotated) of a bacterium and wants to know if it has the potential to cause human diseases, what steps would you recommend...

26 January 2021 8,732 3 View

Can anyone explain why my starters are amplifying sequence from human genome instead of HPV16?

Hello, I'm working on primers from the publication: Okodo M, Okayama K, Teruya K, Sasagawa T. Uniplex E6/E7 PCR method detecting E6 or E7 genes in 39 human papillomavirus types. J Med Virol....

24 January 2021 7,636 3 View

What is your raw statistical treatment preference?

Hi, I have raw data from [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array that I want to process using the Expresso function for Affymetrix microarrays. My samples include tumor...

22 January 2021 1,527 3 View

How to convert ng/ul of DNA concentration to pmol/ul ?

Dear researcher, After amplifying my target gene (1900bp) into the required cDNA by PCR,>cut specific gel bands and purify the gel and the concentration was 30ng/ul. Next, performed A-Tailing...

21 January 2021 2,671 3 View

Can use Unigenes from RNA sequences for overexpression studies (transformation) without requiring a specific plant genome?

Dear researchers: If the plant genome has not been released yet, can Unigenes from RNA sequences be used after BLAST and confirmed with known Arabidopsis genes for function/overexpression...

20 January 2021 5,557 2 View