I get tumor tissue from the patients after the surgery. How to isolate the top layer of cells from a tumor?

I work on gastric cancer. I want to separate the top layer of tumor cells from the lower layers.

25 January 2021 6,663 1 View

How to measure porosity/diameter of electrospun nanofibers using imagej?

Hi all, I have SEM images of electrospun nanofibers and I'd like to measure its porosity/diameter. However I'm not sure how to do it with ImageJ or any other software. Can anybody help me please?...

27 July 2020 5,925 7 View

How important is it to check the psychometric properties of translated version of a gold standard scale?

There is a scale in English that has well established psychometric properties. I want to use the translated version of the scale in my study. Do I have to test all the psychometric properties of...

11 June 2020 3,951 14 View

Should solar irradiance be considered in Wh or kWh when designing a PV system in kW?

Hi, I am designing a PV system in kW and having a small confusion that should I be considering the solar irradiance in Wh or kWh in the PV calculations? Thank you.

02 July 2019 4,127 3 View

Can anyone provide a reliable protocol with tips and tricks for miRNA isolation from human plasma using Trizol?

Trizol protocol I am using, provides good total RNA concentration and quality but the miRNA spike (Cel-miR39) is not being amplified in qPCR. Starting volume: 600microlitre Plasma Elution:...

13 June 2019 2,228 9 View

Can anyone help me understanding why am I getting jagged amplification curve for my miRNAs in plasma sample?

I have used mircury exosome serum/plasma kit along with trizol for extraction of my miRNAs from human plasma. RNA concentration was 623.8ng on nanodrop with 260/280 ratio of 2.12. For RT-PCR and...

11 June 2019 3,679 4 View

What is the best way to purify the ear clip genomic DNA from TaqMan DNA extraction reagent?

13 October 2018 3,427 3 View

Significance of pAkt/Akt and pErk/Erk ratio?

Hi, I'm testing the effect of a drug on cardiomyocytes. The treated cells are then used for Akt and Erk ELISA. Can anyone tell me how do I interpret the pAkt/Akt or pErk/Erk ratio? For...

10 September 2018 3,959 3 View

Insulin concentration in culture medium?

Hi all, to mimic the hyperinsulinemia, what concentration of insulin should be used in the culture medium of growing cell lines (for example L6/MIN6) ? Also what should be the normal concentration...

21 March 2018 2,255 2 View

How to find quantitative determination of fe2+,fe3+ ratio from xps?

In my sample fe deped BaTiO3, both Fe2+ ferrous and fe3+ Ferric ion i.e.,multi valence states present which identified from XPS. How to find fe2+:Fe3+ ration present in my sample? which method I...

20 May 2017 8,210 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View