How is Golgi staining procedure for Drosophila brain?

I look for a robust and simple protocol for Golgi staining of Drosophila CNS. Please share your experience in this matter with me.

09 October 2019 3,414 1 View

What is the meaning of digit written beneath each individual within a pedigree showing genetic linkage?

Hello o everyone There are orders of numbers within a bar beneath each individual of a pedigree representing genetic linkage. Those numbers are corresponding to specific DNA markers. For example...

07 August 2016 4,831 0 View

Why aren't my zebrafish mating?

I have established a new zebrafish lab. At present I face with the problem of fish mating and reproduction. Though the female fishes are mature enough and receive enough food they are not laying...

01 February 2016 2,051 3 View

What are the bases for organization of Eukaryota genome into different chromosome numbers?

There is a huge difference between DNA content of each chromosome. Beside, each eukaryotic organism has specific number of chromosomes. I need to know the possible cellular and molecular bases for...

03 April 2015 3,480 1 View

How to perform a correct and efficient Bi Directional PCR Amplification of Specific Alleles (Bi-PASA)?

Although Bi-PASA is an old method, but in some extend we can use it even nowadays. I need to have knowledge about the points and tips which are needed for primer design. I will be glad if you can...

04 May 2014 9,983 0 View

What are the most recent hot topics in Neurobiology research (especially brain development)?

I am about to wind up my PhD course within next coming 6 months. I need to write and submit a research proposal to get a grant. Since I am interested in brain development using a Drosophila model,...

02 March 2014 1,503 2 View

What would be the order of organism's migration into a newly emerged island?

If a new island emerges in a part of an ocean, what would be the first expected new arrival organisms into the island? I mean the order of changes in that ecosystem which includes migration of...

08 September 2013 4,069 8 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

The most suitable method to calculate the stress intensity factor in the presence of contact between the crack lips?

I'm trying to study cracked structures under a compressive load taking into account contact between the crack lips. I would like to know if the method of extrapolation by displacement remains...

21 February 2021 8,694 4 View

How to assign two different material/section properties in Abaqus(UMAT)?

Hi everyone, I am conducting a non-linear multi-layer pavement structure analysis which is a stress-dependent. So far, I am able to define the nonlinearity for one soil layer for its resilient...

11 February 2021 9,607 3 View

Are there any exception like work hardening in metals elastic limit due to dynamic loading?

The samples made out of spring steel are taken from same lot. The specimens were shaped following heating to forging temperature - Forming- Hardening -Tempering methods. After storage of almost 6...

11 February 2021 952 6 View