What is the meaning of digit written beneath each individual within a pedigree showing genetic linkage?

Hello o everyone There are orders of numbers within a bar beneath each individual of a pedigree representing genetic linkage. Those numbers are corresponding to specific DNA markers. For example...

07 August 2016 4,831 0 View

Why aren't my zebrafish mating?

I have established a new zebrafish lab. At present I face with the problem of fish mating and reproduction. Though the female fishes are mature enough and receive enough food they are not laying...

01 February 2016 2,051 3 View

What are the bases for organization of Eukaryota genome into different chromosome numbers?

There is a huge difference between DNA content of each chromosome. Beside, each eukaryotic organism has specific number of chromosomes. I need to know the possible cellular and molecular bases for...

03 April 2015 3,480 1 View

How to perform a correct and efficient Bi Directional PCR Amplification of Specific Alleles (Bi-PASA)?

Although Bi-PASA is an old method, but in some extend we can use it even nowadays. I need to have knowledge about the points and tips which are needed for primer design. I will be glad if you can...

04 May 2014 9,983 0 View

What are the most recent hot topics in Neurobiology research (especially brain development)?

I am about to wind up my PhD course within next coming 6 months. I need to write and submit a research proposal to get a grant. Since I am interested in brain development using a Drosophila model,...

02 March 2014 1,503 2 View

What would be the order of organism's migration into a newly emerged island?

If a new island emerges in a part of an ocean, what would be the first expected new arrival organisms into the island? I mean the order of changes in that ecosystem which includes migration of...

08 September 2013 4,069 8 View

What are molecular clues to connect oxidative stress and occurrence of neurodegenerative diseases?

Oxidative stress and cellular damage

11 December 2012 9,387 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View