You can stimulate your PBMC with ConA at a sub-optimal dose (0.5ug/mL for my assays, you must check that prolif and cytokine release are still OK). Then, you add your peptide (need to test different doses) to your cells + ConA and compare prolif and cytokine with and without your peptide. You will also need to have a control with your diluent that could be DMSO, glycerol, PBS...to make sure that the effect doe not reflect the one of the vehicle and not this of your peptide...

Cheers

stephanie

Thank you Stephanie, do you know what differences in result I could get if I use either ConA, PHA, PMA or CD3+CD28 to stimulate cell proliferation?

I used only ConA so i cannot tell..sorry

Presumably, using PMA and CD3CD28 you will test the effect only on T cells and not the other immune cells present in the PBMC

Thank s a lot Stephanie.

this depend on lymphocyte stimulates, B or T lymphocytes, in addition to remember above also the LPS also stimulate PBMCs