What are different variant insertion (Gene editing ) techniques used for human cell lines for Functional studies ?

Govardhan Bale @Govardhan-Bale

29 September 2019 1 3K Report

1.Please suggest me full length protocols for conventional and kit based methods

2.My interest is to correlate variant effect on phenotype

3.My plan to work on HepG2 or Huh7 cell line to check the lipid droplet content and inflammation condition

Site directed mutagenesis / CRISPR -Cas9 or any other conventional techniques please provide protocols.

Thanks

Govardhan Bale

Thank you Mohamed Elkablawy

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