Govardhan Bale

6 Questions 10 Answers 0 Followers

Questions related from Govardhan Bale

Tetra ARMS PCR standardization and troubleshooting, what are the optimal conditions and reagent concentration ?

Need the suggestion and help in Tetra ARMS-PCR standardization ... Thanks in advance. 1.What are the optimal/best conditions and reagent concentration? 2.Which one we should standardize first? Tm...

10 March 2022 4,864 2 View

Please share your experience and the Suggestion for Whole Exome data analysis..(for case-control study)

i did whole exmoe sequencing using (NGS) ion Proton system .. i have the patients and control data... need suggestion and the pipelines filters for data analysis how to avoid and know the false...

03 August 2017 1,077 1 View

I want Isolate RNA from stored blood. Can you suggest a suitable protocol?

I have to isolate RNA from 10 ml of -20 stored whole blood. Is it better isolate RNA from whole blood or from PBMCs? Thank you..

10 November 2014 3,833 5 View

Bile salt estimation?

Can anyone recommend a protocol for quantitative estimation of bile salts in bile sample?

06 March 2012 309 2 View

I need a procedure for cholesterol extraction and estimation from biological samples ?

The problem is biological sample having bile pigments, it interfering while estimating the cholesterol..

03 January 2012 2,268 15 View

About ELISA reader .... what is the basic principle ???

ELISA reader at particular nm length absorbs only one color or more ?? EX : at 505 nm length it absorbs pink if i will put yellow color reaction sample its giving high value than pink color. why...

06 December 2011 1,178 3 View