Apart from isolation of endophytic fungi many a times we need to detect fungal endophytes directly into the plant tissue. I would like to ask my peers to shed some light on this query.
Thank you
You can surely try the fluorescence microscopy
Hello Shubhpriya,
Please see:
Chapter Plant Tissue Preparation for the Detection of an Endophytic ...
Article Differential methods of localisation of fungal endophytes in...
Article Endophytic fungal diversity: Review of traditional and molec...
Thank you all.
Regards
Shubhpriya
I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...
02 March 2021 2,735 2 View
We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...
23 February 2021 1,898 1 View
I'm looking to do a sliding wear (plate-on-plate) simulation of GFRP composite plates with Archard Wear Model. Any hints or example steps are appreciated.
17 February 2021 1,447 2 View
There are numerous roots in this tap root system. Kindly suggest or share a research paper where I can clarify the doubts as to which root should be chosen for sectioning and for further root...
14 February 2021 1,816 3 View
We have a AAA class Newport solar simulator. We changed the Xenon Arc Lamp recently (XBO450W, Make:OSRAM) as the lifetime got over. However, the intensity is coming to be only 0.2 sun instead of 1...
14 February 2021 9,998 3 View
I am searching for tools to construct molecular beacons which shall be complementary to a given sequence. I don't want to design any primers. I found that UNAfold is one such tool which I could...
02 February 2021 9,065 5 View
I am synthesizing 7 amino acid long peptide sequence with CTC resin, 50oC coupling for 10 min. On cleavage (TFA/TIPS/Water 95:2.5:2.5) I got peptide with good yield. However, It has 68 Da more...
30 January 2021 8,483 3 View
Hello everyone, I am trying to prepare curvature map in ArcGIS 10.4, using spatial analyst tool. I am using 10 m CartoDem (projected in UTM 44N). I am getting very different value, curvature...
12 January 2021 504 4 View
I want to publish a case report regarding jejunal diverticula
02 January 2021 7,630 5 View
I am trying to maintain two different high DC voltages of same polarity (either positive or negative) between two parallel electrodes separated by 500 um inside the vacuum. However, what I found...
21 December 2020 1,735 3 View
Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...
01 March 2021 1,133 1 View
Incompletely cross-linked pdms are difficult to remove.
01 March 2021 798 3 View
Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.
27 February 2021 9,356 3 View
What is the most suitable medium of ascomycetes fungi ??
26 February 2021 4,413 1 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...
16 February 2021 169 4 View
Is it related to nutrition or any other reason? Any other fungus have similar kind of growth pattern?
09 February 2021 4,357 4 View
Hello everyone, For my research I want to infect Vitis Vinifera leaves with downy mildew-causing fungus Plasmopara viticola. Does anyone know where I could find or purchase this fungi? I am...
04 February 2021 5,720 3 View
31 January 2021 1,832 17 View
I got my fungi sequences back from Illumina Miseq sequencing and I'm wondering how many reads do you use as a cutoff? ie. do you delete anything with less than 100 reads? I can't seem to find any...
28 January 2021 5,646 4 View