Optical direct band gap change with substrate, why?

I deposited Zinc thin films on two types of substrate materials, one is ITO and second quartz substrate. When I calculated optical direct band gap there is difference in energy for 0.2-0.4 eV...

03 April 2019 2,467 8 View

How would you extract lipids without using solvents?

I prepared cell culture with phospolipid of interest. However, in extracting it I shouldnt use any kind of solvent. What techniques would you use that dont have liquid phase for extraction of...

04 May 2018 2,895 6 View

How would you ionized Calcium carbonate without using aqueous solution?

most non aqueous inorganic solvent are poisionos, or they contain protons. What do you think is the appropriate method for CaCO3 disolution without using solvent? some crystals are pyroelectric,...

02 March 2018 1,209 0 View

Why Rolling Circle Amplification is not used more in molecular biology?

We didnt even mention it at my BSc and Msc study in molecular biology, I only heard of it in context of sensor material, dna origami and nano DNA senors. Is this technique new? or it was under...

02 March 2018 7,760 2 View

I have a problem obataing GIXRD results of Alluminium thin film. Why?

I used incidence angle of 1 degree. Aluminium and Aluminium Nitride via sputtering method. I didnt annealed substrates, Which I havent done also with Nickel and Copper thin film, but they showed...

01 February 2018 7,569 3 View

Can you suggest reliable in-situ technique for absorption kinetic of pollutant (heavy metals) to nanoparticles?

I would like to know if there is easy, cheap and straightforward way to determined removal rate of pollutants by nanoparticles? I prepared several types of nanoparticles, (bare, polymer coated,...

31 December 2017 3,779 3 View

How to improve spatial resolution in Computed tomography?

Hello, I wanted to know what is wrong with my CT (CAT scan) image. when I do medical imaging everything look normal and clear but after Multi Planar Reformation picture in sagittal and coronal...

09 October 2017 4,520 5 View

Why different stregnt of magnetic field in magnetron sputtering yield to different results, nitridification rate?

05 June 2017 7,175 3 View

Why wustite ( FeO) and nitride (FeN) have same picks in GI XRD?

Iron nitrides thin films were synthesised, and grazing incidence XRD was applied to characterise crystalline state, Picks confirms structure of FeN, but afterwards I used raman to confirm it is...

08 September 2016 3,261 47 View

Why is sorption enhanced in presence of magnetic field?

06 July 2016 4,359 8 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View