What are differences of different types of multiplex cytokine ELISA?

More Stefan Ručman's questions See All

Optical direct band gap change with substrate, why?

I deposited Zinc thin films on two types of substrate materials, one is ITO and second quartz substrate. When I calculated optical direct band gap there is difference in energy for 0.2-0.4 eV...

03 April 2019 2,623 8 View

How would you extract lipids without using solvents?

I prepared cell culture with phospolipid of interest. However, in extracting it I shouldnt use any kind of solvent. What techniques would you use that dont have liquid phase for extraction of...

04 May 2018 3,056 6 View

How would you ionized Calcium carbonate without using aqueous solution?

most non aqueous inorganic solvent are poisionos, or they contain protons. What do you think is the appropriate method for CaCO3 disolution without using solvent? some crystals are pyroelectric,...

02 March 2018 1,367 0 View

Why Rolling Circle Amplification is not used more in molecular biology?

We didnt even mention it at my BSc and Msc study in molecular biology, I only heard of it in context of sensor material, dna origami and nano DNA senors. Is this technique new? or it was under...

02 March 2018 7,920 2 View

I have a problem obataing GIXRD results of Alluminium thin film. Why?

I used incidence angle of 1 degree. Aluminium and Aluminium Nitride via sputtering method. I didnt annealed substrates, Which I havent done also with Nickel and Copper thin film, but they showed...

01 February 2018 7,722 3 View

Can you suggest reliable in-situ technique for absorption kinetic of pollutant (heavy metals) to nanoparticles?

I would like to know if there is easy, cheap and straightforward way to determined removal rate of pollutants by nanoparticles? I prepared several types of nanoparticles, (bare, polymer coated,...

31 December 2017 3,926 3 View

How to improve spatial resolution in Computed tomography?

Hello, I wanted to know what is wrong with my CT (CAT scan) image. when I do medical imaging everything look normal and clear but after Multi Planar Reformation picture in sagittal and coronal...

09 October 2017 4,679 5 View

Why different stregnt of magnetic field in magnetron sputtering yield to different results, nitridification rate?

I would like to know why I get different nitridification of iron thin film in Magnetron sputtering? by changing for few teslas only, I obtain different percent of Nitrogen in thin film, Is there...

05 June 2017 7,334 3 View

Why wustite ( FeO) and nitride (FeN) have same picks in GI XRD?

Iron nitrides thin films were synthesised, and grazing incidence XRD was applied to characterise crystalline state, Picks confirms structure of FeN, but afterwards I used raman to confirm it is...

08 September 2016 3,419 47 View

Why is sorption enhanced in presence of magnetic field?

Even if you have polymers or any other molecule that can absorb. Presence of magnetic field will enhance sorption. Is this because of influence of mag field to the H2O bonds? The magnetic...

06 July 2016 4,529 8 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Can we use technical replicates for statistical analysis of multiplex cytokine data?

I have done an experiment using luminex technique, measuring 46 analytes. but my sample size is less (N=6, for case and control each). However, i have put up the experimental set in duplicates....

31 July 2024 3,665 3 View

What is the weight of rotavirus A (ng)?

I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?

29 July 2024 3,138 2 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

What do I do with dried urine samples?

I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...

14 July 2024 6,724 0 View

How do I prepare rat cardiomyocyte cell lysate for ELISA via freeze-thaw method?

Anyone have a protocol for making cell lysate from rat cardiomyocyte pellets to run an ELISA using PBS as the buffer?

23 June 2024 2,264 4 View

Ravichandran Panchanathan

Read this paper you will know the difference and advantage about Multiplex cytokine ELISA and Planar array CBA/LUMINEX

Stefan Ručman

thank you very much

Olivia B Harris

I have used milliplex (with the luminex machine) and it was very good for such a large sample size and to look at so many cytokines. However it is VERY expensive for just the plates, let alone the machinery to run it. Hope this helps!

If it is not a secret. How much it cost you?