Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Is iron oxide (rust) a problem in DNA extractions?

Hello, I just started extracting DNA from some spiders, using 5-mm ball bearings to grind the specimens in a tissue mill. I had ordered the ball bearings on Amazon, and thought it was a good idea...

21 July 2020 2,271 6 View

Comparing two variables to one variable- is this possible in SPSS?

In a quantitative study, three variables-- I am comparing two variables (self-concept & well being) with another variable (intent to leave profession). I am predicting to see a positive...

05 May 2020 6,362 3 View

Is there a human gut microbiome database that shows normal variability as well as pathological vs normal ranges for specific microbes?

When a diagnostic lab furnishes normal ranges for certain microbes, is there any place to compare their ranges to what scientists are discovering is normal variability or adaptations? Is there a...

29 December 2019 4,771 2 View

Is it imperative in psychology research to state the null hypothesis?

I have had feedback from a reviewer that I need to restate my hypotheses as null hypotheses. My supervisor disagrees with this suggestion. Having done a bit of digging, I found Szucs &...

12 December 2019 1,447 4 View

How to fit data with binomial distribution?

Hello researchers, Which program or software works best to fit data with binomial distribution? Many thanks, Neena

15 November 2019 6,609 3 View

Stereochemistry Check for Peptides?

Hello researchers, Is PROCHECK the correct tool to check the stereochemical quality of small peptides? My peptide of interest: polypeptide with 16 alanine residues. Many thanks, Neena

28 October 2019 4,653 2 View

I have a GST tagged fusion protein in soluble phase will it have folded?

Normally this protein EIF4E is expressed untagged and expresses in inclusion bodies that are refolded through dialysis, but its very long winded and likes to precipitate during dialysis resulting...

11 June 2019 622 3 View

I am in the process of recruiting participants for my PhD using IPA and am now re-thinking the data collection design. Can you give me any advice?

28 April 2019 8,737 4 View

Approx. how much IL10 do activated T cells secrete after 48 hours in culture?

My students are culturing mixed mouse splenocytes in culture for 48 hours with anti-CD3/28. We are then going to analyse for the presence of Tregs and measure IL10 secretion by ELISA. In order to...

20 March 2019 5,857 3 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View