What are best approaches to study mitochondrial metabolites in chlorine induced lung injury? Are there any publications with other toxicants/drugs?
upon TLR2 stimulation we found both IL1 beta and IL10 were induced why both were induced.
16 November 2020 5,659 5 View
when see names of the cell lines K562 , HL-60 what does the numbers indicate or denote ?
13 August 2019 1,132 3 View
how to isolate platelet exosomes from blood of thrombo cytopenia patients ?
22 September 2017 6,520 1 View
caspases are well known for excutionars of apoptosis but caspases are involved in even more important functions like cell differentiation and proliferation but i think unlike apoptosis where...
14 May 2016 2,828 3 View
I am culturing DAMI suspension cells (Megakaryoblastic) cells in RPMI 1640 (as advised by ATCC formulation, except 2ME). Unfortunately my cells are forming clumps a few hours after seeding (at 1*...
01 January 1970 7,019 0 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
Dear colleagues and scientists: I am asking for your help and insights to develop and explore the feasibility of using an immortalized kidney cell line, for example, HEK293T, for the induction of...
29 July 2024 5,622 3 View
I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...
24 July 2024 5,789 0 View
I intend to get an economical and accurate LAB TEST to measure arsenic concentration in blood sample.
23 July 2024 3,654 2 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...
19 July 2024 3,906 0 View
What is the behavior of anaerobic bacteria in deep salty groundwater with high level of chlorine? Is the nitrate level deceasing and is denitrification controlled by excess chlorine? Thanks
13 July 2024 1,581 4 View
We have been using MFC to control the flow rate of pure ammonia gas. The MFC is placed in an oven at 50 deg. C to avoid blockage due to NH3. However, after a few days of usage, the MFC is unable...
11 July 2024 5,796 1 View
I'm using Dorsomorphin (C24H25N5O) as one component in dual SMAD inhibition of pluripotent stem cells to differentiate into neurons alongwith SB431542. Now as to solubilizing 10mg SB in DMSO for...
11 July 2024 4,832 0 View