how to isolate platelet exosomes from blood of thrombo cytopenia patients ?
Thank you Dinkova
upon TLR2 stimulation we found both IL1 beta and IL10 were induced why both were induced.
16 November 2020 5,659 5 View
when see names of the cell lines K562 , HL-60 what does the numbers indicate or denote ?
13 August 2019 1,132 3 View
caspases are well known for excutionars of apoptosis but caspases are involved in even more important functions like cell differentiation and proliferation but i think unlike apoptosis where...
14 May 2016 2,828 3 View
What are best approaches to study mitochondrial metabolites in chlorine induced lung injury? Are there any publications with other toxicants/drugs?
04 August 2014 3,591 0 View
I am culturing DAMI suspension cells (Megakaryoblastic) cells in RPMI 1640 (as advised by ATCC formulation, except 2ME). Unfortunately my cells are forming clumps a few hours after seeding (at 1*...
01 January 1970 7,019 0 View
Is there any website which can provide free datasets related to project-related data, where details of employee working in a team will be present. I searched in Atlassian Jira and Github .is there...
01 January 1970 3,518 3 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
What should be the zeta potential of bovine milk exosomes
25 July 2024 7,376 5 View
Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...
24 July 2024 9,337 3 View
Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...
24 July 2024 2,158 2 View
Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...
23 July 2024 4,603 4 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
Hi, I need help in detaching adherent monocytes from well plates. I isolated the PBMCs from buffy coat and I did CD14+ positive selection to collect only the monocytes. I let them adhere on my...
20 July 2024 7,922 2 View
These datasets will be used in the training of machine learning algorithms. Does anyone know any available data?"
17 July 2024 6,519 3 View