Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

How or where can I get the crystallographic information file (CIF) for PCN-222 MOF?

I need the simulated XRD pattern of PCN-222 to compare with experimental data. Thanks.

19 February 2021 4,932 3 View

What are the different subclasses in intermediate seeds?

I am currently working on an endemic tree species, that produces mature seeds (moisture content 51%) which shows both vivipary (30% of total harvested seeds from a tree) and dormancy (~40%) of...

20 January 2021 2,670 3 View

Websites for virtual lab in semiconductors?

Hello everyone, Because of Covid, now all classes are online. Is there good website that also are easy to use to teach student virtual experiments about PN junctions and semiconductors? Thank...

24 December 2020 6,637 2 View

From the EDAX analyses if the material which is coated is not observed in maximum percentage then how can interpret the results?

If we doped metal oxide to semiconducting material in different ratios then the property of the resultant material will be increased.But in some lower ratios when we go through the elemental...

16 December 2020 8,190 2 View

Do you can put again my publications online?

Dear All, Please I has accidentaly removed 22 publications from RG my profile in the mail that you send me today, please put again those publicatios in my profile. Best regards,

13 December 2020 9,981 4 View

Is there any software for calculating hit bias (HB), missed precipitation (MP), and false precipitation (FP)?

I have a time series of two rainfall products. For error decomposition analysis, I have to calculate HB, MB and FB. Is there any software for calculating these variables.

27 November 2020 980 2 View

Why Schiff bases change colour upon evaporation?

I got bright yellow coloured Schiff base after 6 hour reaction time. So i kept it for slow evaporation. Solvent was methanol. But after complete evaporation my product colour changes from yellow...

17 November 2020 3,824 2 View

How to make the children cool, who feel stressful during this pandemic-lock down without having physical outlet?

The discussion may feed the parents a way to manage and guide the children in a right direction.

12 November 2020 1,302 9 View

How to design qPCR primer for mature microRNA?

09 November 2020 9,163 3 View

How is it possible to correct the pH of landfill leachate permanently?

Hi Good afternoon everyone Can someone help me please? We are trying to correct the pH of a landfill leachate treatment plant. The pH of the effluent is 9 and we are trying to make the correction...

14 October 2020 4,775 10 View

Which environmental problems arise from landfill mining ?

Landfill mining techniques arise as one of the available alternatives to mitigate negative environmental impacts from municipal solid waste landfills. High recycling potential generation is one of...

28 March 2020 1,782 10 View

Could airborne microplastics play a role in climate change?

02 March 2020 8,981 5 View

Could airborne microplastics play a role in climate change?

02 March 2020 454 11 View

Is there a recommended article on RAMAN Spectroscopy and/or FTIR that also works as a guide?

Fellow scientists, I'd like to know if a test solution contains certain leachates, meaning that an extraction method I am developing works. Much appreciated beforehand.

13 January 2020 5,249 3 View

Why did my LR White treatment not work efficiently?

This picture is from Arabidopsis thaliana leaf cells treated with LR White resin. As you can see cracks and shrinkage in the cells, which I think it was caused due to insufficient dehydration and...

10 November 2019 2,965 3 View

Is data on "number of landfills" in Indian cities available?

City wise data for number of landfills. For example, number of landfills in Delhi = x, number of landfills in Mumbai = y, number of landfills in Agra = z.

09 October 2019 7,063 3 View

Accuracy assessment of landfill site suitability classes using error matrix?

Could anyone help me to explain either we can or cannot do validation by using landfill site suitability classes (Unsuitable, Low suitable, moderately suitable, highly suitable and Very Highly...

08 September 2019 4,520 1 View

What is the sinkhole mechanism under a leaked sewer pipeline in a non-krast area (areas with no carbonate rocks or lime stone)?

What is the sinkhole/subsidence/ ground collapse mechanism under a leaking sewer pipeline in a non-krast urban area (areas with no carbonate rocks or lime stone)? Because sinkhole also use to...

07 August 2019 7,279 6 View

What is effect on the settlement rate of soil, when water infiltrates continuously and when infiltrates non-continuously (cyclic)? WHY?

What is effect on the settlement rate of soil? First when water leaks from sewer or water pipe leaks continuously (non-cyclic flow) from an underground pipeline? Secondly when the water from...

06 August 2019 3,081 4 View