Im trying Candida selection in Sabouraud %2 Dextrose Broth. I need inhibit oral bacteria. What antibiotic concentrations should be used ?
Possibly, Vancomycin 7.5 mg/L for Gram positives , what about Gram negatives? and in which concentration is advised?
More than just a single antibiotic I would use a cocktail (for example you can use clindamycin, metronidazole or ciprofloxacine) to asure large decontamination of the whole oral microbiota
Thanks for answers
Maxime can you please advise me some literature sources to quantify which antibiotic concentrations can be used
You can use chloramphenicol to inhibit the growing of gram positive and gram negative bacteria. The usual chloramphenicol concentration is 0.05 g/L.
I would use an oral rinse, followed by centrifugation and direct planting onto a selective agar such as Sabouraud + 2% dextrose (pH 5.6 - acidity inhibits many bacteria). This medium is available commercially with or without chloramphenicol, which makes it even more selective. Instead of Sabouraud you can also use CHROMagar Candida https://www.ncbi.nlm.nih.gov/pubmed/7989544
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