For investigating mercury bioremediation by bacteria, consider using SEM-EDS for structural analysis, AAS for quantifying mercury, and GC-MS for identifying transformation products. Additionally, techniques like fluorescence spectroscopy, electrochemical methods, and microbial community analysis can provide deeper insights into the underlying mechanisms.

When discussing heavy metals bioremediation, we target the reduction and biotransformation concepts instead of biodegradation. the underlying mechanism can be studied by targeting gene-specific studies (PCR technique); differential expression (rtPCR; whole genome analysis), biosorption and bioaccumulation by AAS/ICPMS; localization of metals by SEM-EDX; TEM-SDEX; fluorescence microscopy, etc. The biotransformation of Hg by powder XRD and its analysis using software like MAtch and QualX, involvement of biomolecules or HM effects on metabolism by Omics study.

I'd suggest that you consider where the Hg is going to

Hello Dr. Paul Milham! Could you clarify what you meant by your response?

Danny Jr. Honorio ,

This might involve knowing how Hg transportation occurs in bacterial cells via amplification and identification of mer genes and quantification by rtPCR. Studies on intracellular and extracellular ionic fluxes (pH) also help to understand Hg transportation in the bacterial cell.

Hello,

Your analytical approach covers a broad spectrum, and the techniques you've proposed are highly effective for understanding different aspects of mercury bioremediation.

Here are a few additional methods to complement your research:

These methods can provide a deeper understanding of how mercury interacts with bacterial cells and the transformation processes it undergoes.