Dear Sneha!
Please You look at this protocol:
Article Biocomposite nanofibrous strategies for the controlled relea...
Expression of F-actin
Figure 11 Laser scanning confocal microscopy images. Notes: Immunocytochemical analysis for the expression of F-actin staining with rhodamine-phalloidin (B, E, H, K, N) and (C, F, I, L, O) overlay images of (B, E, H, K, N) on TCP (B, C), PLACL (E, F), PLACL/SF (H, I) PLACL/SF/VE (K, L), and PLACL/SF/VE/Cur (N, O) with the nuclear staining by DAPI (blue fluorescence) at 20× magnification. Nucleus stained with DAPI (A, D, G, J, M).
Sneha K Ramanathan By cytocompatibility which parameters do you mean? If it's simply viability and cell morphology, then DAPI gives a decent insight into whether the cells are healthy or apoptotic or necrotic. Nuclear blebbing and fragmentation in unhealthy cells are commonly observed in cells when the material added is not compatible. Actin filaments stained with phalloidin can give a precise qualitative measure of the cytoskeleton assembly of the cells. If the material is not cytocompatible, you would see altered cell morphology and abnormal cytoskeleton arrangement. If your nanomaterial is fluorescent-labeled, you can also visualize its internalization in the cells using confocal microscopy. The quantitation of nanomaterials inside cells can also be performed using ImageJ particle analysis. Hope I could be of some help. All the best!
- Badges
- Science method
- Similar topics
- Biological Science
- Histology
- Staining