I am performing invitro bioactive test for polymer scaffold. Is it necessary to change the SBF medium every week
I thinkthat really depends on what you are doing within the test. If you are doing releasing test, you might need to replace the media at pre-determined time intervals. If you are co-culturing scaffolds with cells, you may need to replace the SBF.
In general, it is recommended to change SBF (simulated body fluid) every week when using it to test the bioactivity of scaffolds in vitro. This is because SBF can become depleted of certain ions and minerals over time as they are absorbed by the scaffold or react with other components in the solution. By changing the SBF on a regular basis, you can ensure that the solution remains physiologically relevant and supports cell growth and differentiation.
However, the frequency of SBF changes may depend on the specific experimental setup and the desired outcomes. For example, some studies may change SBF every two or three days instead of weekly to maintain a consistent chemical composition. Other studies may only change SBF after a longer period of time, such as two weeks or more, if the scaffold is able to maintain its structural and chemical properties over a longer period of time.
Therefore, the optimal frequency of SBF changes may vary depending on the specific scaffold and experimental setup, and it is recommended to consult the relevant literature or consult with other experts in the field to determine the best approach.
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