We are trying to isolate dorsal root ganglion neurons from guinea pigs. Can anyone suggest the age of animal to go for which may be easier to dissect and give nice quality and purity of cells? Thanks for any inputs! =)
Filipa Franquinho
My experience is only in isolating adult DRG neurons from both mouse and rat. Usually the cultures are not extremely pure (glial cells might account by 30-50% of the total cells) but the neurons grow very well and are much easier to isolate.
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Katie Cheung
Thank you very much for your information! That's very helpful! Seems it's more common to use mice and rats for the isolation. Did anyone have any experience with guinea pigs?
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