How to calculate age-standardized prevalence ratio with 95%CI?

I need to calculate the prevalence ratios to show the trend of prevalence of a drug group used by pregnant women over the years (2001-2018). I would like to use the year 2001 with age distribution...

05 January 2021 7,986 5 View

Does CD31 indicate cell viability?

Hi, I am wondering if the immunofluorescent staining of CD31 antibodies can both mark endothelial cells and indicating their viability. In other words, if I perform immunofluorescent staining for...

23 December 2020 5,815 4 View

How to increase titer of Herpesvirus, Adenovirus?

I'm working with Herpesvirus and Adenovirus. For Herpesvirus, I use Vero cell line to activate and check virus titer. For Adenovirus, I use Hela cell line. Recently, I could activate them, but...

16 December 2020 4,073 5 View

How big can a enhancer deletion be ? how can we obtain homologous deletion clone ?

Hi all, I have tried to use Crispr Cas9 ( plasmid px333_cas9_mCherry) to delete some enhancers. I designed sgRNA using Chopchop website to target both sides of an enhancer to induce homologous...

13 December 2020 942 3 View

How to calculate the fractional vegetation cover (FVC) from Landsat imagery?

Hi folks, I wonder if anyone know how to calculate the fractional vegetation cover (FVC) from Landsat imagery? As far as I have read about that NDVI, NDVIs, and NDVIv are three required input...

07 December 2020 4,054 2 View

Is it possible to identify/classify rubber using satellite imagery with machine learning?

As of now, I am trying to explore the potential of using high/very high-resolution satellite imagery with machine learning (ML) to identify or map forest cover (e.g. rubber). As a newbie in this...

17 November 2020 7,187 3 View

Cloudy brain samples in sucrose solution?

I have some samples I left in sucrose for a couple of weeks after perfusion and was wondering if these samples are still salvageable? I perfused the brain, then they were fixed in 4% pfa for 24...

15 November 2020 1,728 2 View

CDNA PCR problems: why did the sample without reverse transcriptase enzyme (band 5 in both two genes) have strong band with gen A?

Dear all, These are data from gel base of cDNA sample for PCR with protocol attached on the figure. Band 5 is no reverse transcriptase enzyme during doing cDNA library used random primer. With gen...

04 October 2020 8,774 4 View

Why were cDNA PCR got multiple bands when added DMSO?

Dear all, All samples are cDNA, using reverse transcriptase enzyme to make cDNA from RNA. One group is doing PCR without DMSO, and other group is doing PCR with DMSO. One is without DMSO, the...

04 October 2020 1,320 3 View

Beta-metacaptoethanol in Sample Buffer (Western Blot)?

I'm working on Western Blot and was guided to use the Sample Buffer Solution (2ME-) (x4) (Fujifilm Wako). However, Beta-metacaptoethanol (BME) is said to be necessary to cut the disulfide bond in...

13 September 2020 5,533 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

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02 March 2021 3,060 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View