I have used up my snap frozen tissue from an experiment but have some left in RNAlater (invitrogen).
I was wondering if anyone had any experience with running western blots using tissue that has been stored in RNAlater?
I haven't done this but from the limited research I looked up, it seems possible. The quality of the proteins seems to be tissue dependent though.
https://www.thermofisher.com/ca/en/home/references/ambion-tech-support/rna-isolation/tech-notes/rnalater-for-rna-and-protein-stabilization.html
https://www.researchgate.net/post/Does_anyone_have_experience_with_extracting_protein_from_RNALater_stored_tissues
Article Simultaneous Evaluation of the Preservative Effect of RNA la...
I've some experience with mice hearts in RNALater. For me, the tissue was "rubberized". I think muscular nature of this tissue exaggerates this effect of RNALater. Was hard to extract protein with the 15, 18 and 21 gauge needle so i wouldn't recommend this as a method. However this method worked fine for RNA with good yield. I ended up using tissue homogenizer + RIPA buffer to get protein with good yields.
Hi Olivia Fay Matthews ,
I did this extraction , but it does not work well. The tissue is hard to disrupt and protein hard to ressuspend.
Best,
Jacó
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