Olivia Fay Matthews

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Questions related from Olivia Fay Matthews

Western blots on tissue which have been stored in RNAlater?

I have used up my snap frozen tissue from an experiment but have some left in RNAlater (invitrogen). I was wondering if anyone had any experience with running western blots using tissue that has...

29 August 2019 8,068 3 View

What are the best conditions for transferring a >200kDa and ~40kDa protein?

I am having issues transferring my large protein (220kDa) at 30V for 2 hours (RT). It is often patchy with incomplete bands (annoyingly I occasionally I get a clear transfer!). For my other...

23 August 2019 1,273 7 View