Western blot or ELISA for quantifying antifreeze proteins (AFPs)?

03 October 2023 1 10K Report

Kind and warm greetings to all

I'm working on Antifreeze proteins(AFP) that is expressed by Yeast So which method \ assay is more reliable and effective to detect AFP by western blot or ELISA with references ? and why? Kindly if anyone working now or previously on Antifreeze protein can you guide me.

Abhishek Jaiswal

ELISA (Enzyme-Linked Immunosorbent Assay):

Advantages: ELISA is highly sensitive and can provide quantitative data about the concentration of the anti-freeze proteins. It is a widely used method for quantitative protein analysis.
Limitations: ELISA requires specific antibodies and may not be able to differentiate between different isoforms or modifications of the anti-freeze proteins.

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