Thank you Ahed, the first link I'm able to catch, the next (http://stke.sciencemag.org/cgi/reprint/6/274/ra32), I'm unable to open. Could you plese give me title or PMID of this article?

anyway next articles are desired;)

are you looking for a p53 WB-AB for mouse or human because that's quite a big difference.

I can tell you about our experience with human / mouse leukemia cells.

For mouse you can use the one from Cell Signaling which works fine. Just look it up on their website.

For human after extensive searching the one which works well in our hands is one from BD: CAT 554294 which gives though a VERY strong band and thereby i suggest that you really do it after you may have already stripped your sample for beta-actin.

Best

Dear Behzad, thank you a lot! I want to perform western on human head&neck cancers means all the possibilities in fact. All the details, your references would be appreciated! Just please do note I already have found a lot of procedures done on cells grown in incubator. I want to have the procedure for the material isolated from living human/animal. Many thanks again - Jan

Dear Jan,

we use primary xenografts from patients BUT they are kept in culture. I'm to be honest not familiar with directly isolated primary cells but i would suggest to regularly use lysis protocols for solid cancer types. I think you will find many lysis protocols if you pubmed for them or ask around. The protocol thereafter are a regular western blot protocols from a cell pellet. although you may rather want to consider histological slides from your solid tumors as far as this may be possible as a secondary option.

The BD AB (for human), I mentioned was used in a recent reference from our lab:

http://www.nature.com/nm/journal/v19/n8/pdf/nm.3247.pdf

Figure 4c

Best,

Behzad