Weird plasma RNA profile after miRNeasy Serum/Plasma Advanced extraction kit by Qiagen?

Jennifer Pérez-Boza @Jennifer-Perez-Boza

09 February 2022 1 660 Report

We have performed RNA extraction from several samples of plasma with miRNeasy Serum/Plasma Advanced and in 1:4 samples we get this strange peak at around 3000nt. Has anyone seen something similar using this kit?

Thank you.

Julie Ann Dougherty

are you doing DNase digestion? might be DNA contamination

are you diluting the sample prior to isolation? sometimes all the salt in serum/plasma can mess with isolation. I would dilute 1:4 -1:10 in PBS then elute in a small volume.

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