Weird plasma RNA profile after miRNeasy Serum/Plasma Advanced extraction kit by Qiagen?

Jennifer Pérez-Boza @Jennifer-Perez-Boza

09 February 2022 1 664 Report

We have performed RNA extraction from several samples of plasma with miRNeasy Serum/Plasma Advanced and in 1:4 samples we get this strange peak at around 3000nt. Has anyone seen something similar using this kit?

Thank you.

Julie Ann Dougherty

are you doing DNase digestion? might be DNA contamination

are you diluting the sample prior to isolation? sometimes all the salt in serum/plasma can mess with isolation. I would dilute 1:4 -1:10 in PBS then elute in a small volume.

Badges
Science topic

More Jennifer Pérez-Boza's questions See All

When should I stop watering A.thaliana for collect their seeds?

I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...

30 July 2024 2,301 7 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

How do I identify And count the number of active sites of an adsorbent?

Jarosite is obtained from bioleaching residue and I want to determine if there are increased active sites biogenic jarosite compared to chemical synthetic jarosite.

10 July 2024 8,132 2 View

What scale can I use to measure consumer's trust with marketing activities?

Scales?

10 July 2024 1,176 2 View

What’s the cost of maintance of inactive coal fired plant of 1500 a mw with a chemney of 354 meter high?

One plant was closed it is uncertain what would be the cost of maintaining the infraestructure as industrial heritage. Any idea?

09 July 2024 7,969 0 View

Hello, could you please share how you measure biomass/growth of Aspergillus oryzae in your lab?

Hi, I would like to gain an insight on the various methods of measuring biomass and growth of Aspergillus oryzae, which you have personally tried and would gladly recommend. Thanks.

08 July 2024 6,057 2 View

Any automatic way to outline an image to measure the area in pixels?

Hi, I am trying to measure the area of the caudal fin in zebrafish, to study regeneration. I need to measure the area of the regenerating fin every 3 days after injury. So far, i have been using...

27 June 2024 5,068 1 View

Can anyone share the JCR impact factor for 2024?

Please can someone share the list of journals with impact factor published in 2024?

27 June 2024 2,927 0 View

What is the most pressing agricultural setback to post harvest produce?

The major causes of post harvest loss of produce in Ghana.

20 June 2024 6,445 5 View

¿En qué medida las tecnologías educativas están siendo efectivas para promover el desarrollo de habilidades para la vida?

las

19 June 2024 2,125 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View