We want to grow MCF10A cell lines. However, it is really difficult to get cholera toxin in Hong Kong, which is one component of the medium for MCF10A. And I checked references, it seems that without cholera toxin, MCF10A will grow really slow. Since we cannot get cholera toxin easily, is there any alternative way to replace cholera toxin and grow MCF10A in a good way?
What do you mean you cannot get chlolera toxin easily? Get it from Tin Hang technology, the distributor of Sigma product in Hong Kong.
https://www.sigmaaldrich.com/catalog/product/sigma/c8052?lang=en®ion=HK
We asked this distributor and salesman said in order to get cholera toxin we had to prepare export and import document. And they have a current case which already took more than 6 months to process it and haven't finished.
Dear,
How about the isoproterenol?
Curr Eye Res. 2012 Jul;37(7):644-53. doi: 10.3109/02713683.2012.669510. Epub 2012 May 4.
Effects of isoproterenol and cholera toxin on human limbal epithelial cell cultures.
as
""Addition of cholera toxin to human mammary epithelial cultures derived from reduction mammoplasties and primary carcinomas greatly stimulated cell growth and increased the number of times the cells could be successfully subcultured. Other agents known to increase intracellular cAMP levels were also growth stimulatory. The increased growth potential conferred by cholera toxin enhances the usefulness of this cell culture system."
Stampfer, MR. Cholera toxin stimulation of human mammary epithelial cells in culture. In Vitro. 18: 531-537. 1982."
From https://www.researchgate.net/post/Is_there_anyone_working_with_the_MCF-10A
Good luck
Dear Jinbiao,
Thank you for your help! I will try to use isoprenaline, but just worried about how other researchers may accept it or not.
Thanks again!
Best wishes,
Sdiong
Sidong, I am quite surprise that application for import took so long. Maybe you can try other distributor (different brand). Tin Hang has the reputation of slow processing time.
You may try
https://www.caymanchem.com/product/19654
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