I would like to know what else need to be added to make complete medium that can be used in 5% CO2 incubator, do we have to add Non Essential Animo Acids and Sodium Pyruvate, and HEPES apart from sodium bicarbonate? and how much amount Sodium bicarbonate needs to be added to prepare 1L medium to be used in 5% CO2 incubator. To be more specific, Gibco Minimum Essential Medium powder, catalog number 61100-061
Sodium bicarbonate should be added at 2.2 g/liter to achieve 26.2 mM final concentration.
Muruganandan Shanmugam , Thank you so much Sir for your response, I would also like to know about Non-essential Amino Acids and Sodium Pyruvate. Will be waiting for your reply, exact product that we purchased is Gibco Minimum Essential Medium powder, catalog number 61100-061, description in the below link
https://www.thermofisher.com/order/catalog/product/61100061
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