Offlavor pond fish and usage of bio-organic agent ?

Impressive sharings! Regarding on that matter,somehow people use kind of tamarind whichever in paste or liquid to get rid offlavor on flesh fish,wondering is it live fish can be done that way...

11 April 2020 2,155 3 View

How does R programming language add non numeric operators?

Say I have list x(a vector of character names) and list y(a vector of data). I want to run each element of data within y through a loop, but I want it to print the element from vector x that has...

06 April 2020 7,867 3 View

Suggestions for immunofluorescence of rat spinal cord?

I am having difficulty getting specific binding with my rat spinal cord immunofluorescence. My protocol is as follows: 1. Remove spinal cord and quick freeze in TissueTek OCT. 2. Cut tissue on...

03 September 2019 9,215 2 View

What did my western blot fail???

I am a beginner in western blot and the results were okay for the last few weeks with beta actin as housekeeping control. The transfer buffer ran out a few days ago so I prep a new 10X transfer...

13 February 2019 3,274 9 View

Should we use a Boneferroni correction to adjust the sgnificance level when performing Wilcoxon statistic for multiple pair comparisons?

I will perform a Friedman analysis on three means (a, b, c) from the same sample. If the Friedman is significant, I will perform a Wilcoxon as a follow-up test to assess which mean is different...

30 May 2017 8,236 10 View

Where can i get the specifications for the different layers that make up tire?

here is paper i wish to replicate before i get into my thesis

03 February 2016 4,211 4 View

Why am I getting crooked bands in SDS-PAGE?

Why am I getting this type of pattern? I am thinking the problem is either the running buffer or the sample buffer. I have changed new running buffer but the bands are still crooked. Could the...

30 January 2015 8,216 11 View

What is the reason for fungs appearing in the crystal growth system when conducting slow evaporation?

01 January 2015 5,354 3 View

I have recently generated 5 Kb genome fragments from cDNA and would like to sequence these fragments. ABI BigDye isn't working. Any ideas?

I have sequenced with ABI BigDye many time in the past but now am getting junk from my direct sequencing reactions.

31 January 2013 7,575 18 View

Can We Collaborate?

Hi, Can your collaborate with us at the University of Lagos. Our University has just approved a Gemonic Center for Non-Communicable disease and Cardiovascular diseases including Congenital heart...

01 January 1970 3,987 31 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View