How does fluent FWH solve acoustic data at a location where no mesh is present?

In the simulation tutorial of the aeroacoustics around a cylinder by ANSYS, recievers are placed at a location where mesh isn't present, how does the solver predict the acoustics at that location...

16 October 2020 3,368 5 View

Problem with Cryosections?

Hi, I have a problem with cryosections while staining. Positively charged slides are used for cryosections, in spite of that during the staining process, the sections are washed away during the...

10 September 2020 3,767 3 View

Can we use 2, 6-dichlorophenol indophenol (DCPIP) method for analyzing crude oil degradation containing high sulfur content in the crude oil?

I was trying to find out whether my bacteria can degrade crude oil or not using DCPIP method that changes color after oxidizing hydrocarbons. But, I happen to order the crude oil containing 5%...

25 June 2020 9,801 2 View

Why my lenticrisprv2 plasmid after ligation of oligos is of smaller size than the expected?

after miniprep, I sequenced the plasmid. After sequencing I found that a region of plasmid was missing and its size if of around 8kb while it should be of around 12kb. It has happened to me for...

21 March 2020 736 3 View

HnE Protocol for the frozen section of cells encapsulated in hydrogels?

Hi all, I'm facing problems in staining the cryosection of the cells encapsulated in hydrogels. I'm using both charged slides as well as the albumin coated slides for the HnE staining. During the...

28 May 2019 1,197 5 View

Has anyone done co-transfection with siRNA and the fucci system viral vectors by Thermofisher (Cat: P36238) ?

I'm trying to knockdown my protein in adherent cells and label cells with the fucci system for cell-cycle segmentation using microscopy. My protocol is as follows Day 0 : siRNA...

07 August 2018 4,362 4 View

Can anyone suggest me some ph.d research topic from machine learning and deep learning?

I am currently pursuing Ph.d

01 January 2018 7,222 17 View

What is the procedure for performing ELISA on protein pellet extracted from cell lysate?

Hi all, I wanted to perform ELISA (sandwich elisa) on the protein pellet extracted from cell lysate. I would like to know in which buffer, the protein pellet should be resuspended? Thank you Nisha

10 December 2017 9,325 3 View

Acceleration time history for Korba earthquake in India?

Can I get it in matlab format or notepad

08 October 2017 7,788 3 View

Where can I find a dataset suitable for Recommending Learning Materials to the students?

I am interested to start a project for recommending learning materials to the e-learning_students. Can I find any dataset which can be used for training an algorithm based on the learning style...

08 October 2017 7,627 6 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View