Does anyone have a suggestion on how to properly titrate virus after inactivation and purification step? I was planning to inactivate virus using chemical protocols (such as formalin), purify by cushion ultracentrifugation, but after all steps, the only way I figured out to quantify my virus particles was by ELISA assay. Any clue how to titrate more precisely??
Thank you so much
After inactivation, you can quantify the virus by determining the total protein concentration by conventional protein estimation methods. The other method of quantifying the inactivated virus is by qPCR. qPCR will be a robust and sensitive method.
Best
It is really depending on the virus you are using; for example for influenza you can use the Single Radial ImmunoDiffusion assay. Indeed qPCR can be used too, just use the right control for quantification.
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