Vector Nti program???

More George Rafael Samantsidis's questions See All

No colonies in typical cloning- any feedback?

Hello everyone, I am trying to construct a vector in order to study promoter activity with YFP reporter gene. The final vector is going tobe injected in insects. The cloning strategy is based on...

07 August 2018 4,737 10 View

Does anybody know what are the best conditions for getting a pellet in oligos precipitation with ethanol?

I used glycogen as a carrier and NaAc for neutralization but I'm not able to see the white pellet every time. The concentration of the DNA is 0.0086 fmol/ul.

02 March 2014 8,363 4 View

Knockout mutant in bacteria

Does anybody know how difficult is to create a knockout mutant in a plasmid or in the chromosome of Bacillus thuringiensis?

06 July 2013 1,861 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,229 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View

Isolation of PLV6-Bmal-Luc plasmid?

I am currently at a stage in my research where I will be using the pLV6-Bmal-luc plasmid (Addgene #: 68833). However, I am encountering some issues with the isolation of this plasmid and am unable...

23 July 2024 5,296 0 View

Daniel Prieto

You could take a look at serial cloner. You will not be cracking anyone's stuff, and you will not be stuck into proprietary formats.

Best of luck

http://serialbasics.free.fr/Serial_Cloner.html

Athanasios Siametis

Check a few more answers about this thread:

https://www.researchgate.net/post/Plasmid_drawing_program

https://www.researchgate.net/post/Free_software_or_website_similar_to_vector_NTI/1

George Rafael Samantsidis

Thanx mister! :P