Vaccination on currently covid 19 asymptomatic person?

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What to do for Error termination via Lnk1e in /share/apps/gaussian/g16/l502.exe, for Ce through B3LYP/SDD?

I am trying for the structure optimization via Gaussian16 DFT calculation, for a system consisting of C,H, N, O and Ce atoms. Where I am using B3LYP/ 6,311G(d,p) C, H, N and O and B3LYP/SDD for...

30 June 2024 9,979 4 View

What basis set/basis sets will be useful for DFT (using Gaussian16) of cerium ion adsorption?

24 June 2024 6,927 3 View

Any specific peptide based ligand for mitochondrial targeted delivery of nano-drug delivery systems?

Suppose I need to target mitochondria to address malignancy-related problems. Are there peptide-based ligands similar to Nuclear Localization Signals (NLS) for nuclear targeting that can be used...

20 June 2024 9,587 1 View

How to perform Fmoc protection to amino acid with Fmoc-osu?

Kindly mention the protocol

27 May 2024 3,243 0 View

Force field parameters for TIP4P-Ew (Oxygen - Dummy atom)?

What are the force field parameters for TIP4P-Ew water model? Including the force constant of Oxygen-dummy atom bond length, and hydrogen-oxygen-dummy atom angle? So, basically, what is the...

08 April 2024 3,426 0 View

Can we transfect macrophage cell (RAW264.7)? is it easy to transfect?

kindly provide some insights

01 April 2024 4,674 2 View

What can be the suitable media for culturing U-87-MG?

To culture the U-87-MG cell line, various media formulations are available online. These include: a. Essential Minimum Eagle Medium (EMEM) supplemented with 10% fetal bovine serum (FBS), 100 U/mL...

07 January 2024 612 0 View

Why is U-87-MG cell line not showing growth?

For the past few weeks, I've been attempting to passage U-87-MG cells in our lab. However, after thawing, instead of reaching 80% confluency within 24 hours, they're taking 3-4 days to achieve the...

21 December 2023 509 2 View

Can someone suggest a protocol for isolating RNA from whole blood?

I have used Trizol method but there has been huge DNA contamination along with shearing.

17 December 2023 4,745 4 View

Is it possible to detect emission in the visible range using ED-XRF or WD-XRF equipment utilizing x-ray excitation?

I understand that ED-XRF or WD-XRF only allows for high energy photon emission. I am wondering if it is possible to obtain emission in the visible region by attaching an external optical fiber to...

29 November 2023 2,939 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

E-commerce businesses, COVID-19 and Entrepreneurial Strategies (innovation, proactiveness and risk-taking)?

Hello: I am looking for articles that address e-commerce businesses and COVID-19 survivors, as well as Entrepreneurial strategies that reflect e-commerce businesses. Blessings Evelyn Dempsey

05 August 2024 5,332 3 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

(treating astrocytes with LPS) Has anyone observed the recovery of astrocytes from LPS injury, a few hours after the treatment?

or can you recommend articles showing the differential effect of LPS in a time dependant manner?

28 July 2024 2,547 1 View

Why not we are following sars COVID 19 guidance?

If the case of Irak is of December 2020 then i think I can suggest pl follow all the infection cause and death, already in our researchgate.

19 July 2024 3,356 2 View

Size-biased recovery of total RNA isolated with Trizol vs RNeasy?

So - my lab did a bunch of RNA isolations from tissue culture cells, for purposes of RNA-seq, and submitted them to a core facility. We got back a notification that the RINs were too low, and we...

14 July 2024 641 1 View

Solvent recovery after extraction - Separation of Isopropanol + Water - how to do it best?

I want to make some yeast extractions with a protocol that uses Isopropanol and NaOH. So in the end i will have an azeotrope of Isopropanol and Water that comes from the cell and NaOH. Can you...

08 July 2024 9,201 2 View

How would I calculated ddPCR spiked nucleic acid recovery calculations?

Hello everyone, I'm in the initial stages of conducting a study involving the ddPCR (Bio-Rad QX200, QX600) and I ran into some problems involving recovery % calculations using direct...

02 July 2024 6,011 2 View

How to load Remedesivir drug into mesoporous nanoparticles?

Hello, I want to load Remedesivir (RNA polymerase inhibitor) into mesoporous nanoparticles (MSNs). The issue is drug is temperature and pH sensitive and it degrades quickly. I need advice on its...

01 July 2024 889 3 View

How does neuraxial anesthesia impact postoperative recovery compared to general anesthesia?

The Evolution and Application of Spinal Anesthesia: Techniqu... How does neuraxial anesthesia impact postoperative recovery compared to general anesthesia?

27 June 2024 4,159 1 View