13 March 2023 3 3K Report

Want to do migration assay in T047 D cell line , please suggest protocols

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Doping of metal on noble metal nanoparticles?

can we dope any metal (Cu, Zn, Mg) on silver or gold nanoparticles and how can it be done ?

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How do i calculate sample size for a survey questionnaire of my research?

I am working on tobacco intervention and developing communication strategy, for that a questionnaire was developed, population is Indian tobacco users.

13 April 2024 9,324 2 View

What is accurate methode to set up two electrode device in supercapcitor device?

I made two electrode devices for the supercapacitor. I combined activated carbon and PVDF for negative electrodes in an 80:10:10 ratio. I combined active working material, PVDF, and activated...

12 February 2024 2,964 3 View

How to import file on xpspeak41?

I want to analyse XPS spectra from xpspeak41. I try every file format such as ASC, .dat but it always shows an error reading file. what should I do? please see the images I attached below

11 February 2024 6,860 3 View

How can I make different concentrations of antigen solution from a stock?

I have 50 ug of Human Her2 / ErbB2 Protein, Tag Free antigen in white powder form and need to make various concentrations of that antigen, such as 50 unit/ml, 10 unit/ml, 0.01 unit/ml, and 0.1...

05 February 2024 9,048 6 View

Can someone please help me with optimized protocol for intracellular melatonin detection using fluorescence spectroscopy ?

My work involves the production of melatonin using saccharomyces cerevisiae and its quantification. I have tried various fluorescence spectroscopic methods but couldn't find an optimized protocol...

15 January 2024 9,410 3 View

I want to design primers for RT-PCR, how many primers can be multiplexed in one go?

For validation studies, I want to use RT-PCR for genes derived from sequencing data. For that I have to multiplex the reaction to see the expression for more than one gene. How many primers can be...

18 December 2023 8,387 1 View

How to quantify intracellular melatonin without using HPLC or Mass Spectroscopy ?

I am currently working on yeast cells for melatonin production which requires me to quantify melatonin easily and economically. I was hoping that anyone could provide me with an optimized protocol.

09 December 2023 7,037 1 View

How can I speed up the growth of single MCF-7 clones after cell sorting?

I recently did a single cell sort with MCF-7 cells to create my CRISPR cell line. I sorted single cells in a 96-well plate in MEM media with 10% FBS, 10ug/mL insulin, and 1mM sodium pyruvate. It...

28 October 2023 6,320 1 View

Why is there often a difference in the values of specific capacitance measured using Cyclic voltammetry and GCD? Which value should I use?

Which value is more accurate and why?

17 October 2023 1,418 0 View

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