What Contaminates Cause Loss of Resolution in Plasmid Sanger DNA sequencing?

We use an AB 3130xl Genetic Analyzer for Sanger Sequencing, and on occasion, we see our plasmid samples struggle with wavy, loss of resolution (as seen in the attached image). Our general...

14 January 2021 7,346 6 View

Disappearing Coomassie Bands?

Suddenly my Coomassie staining has produced strange results (see photo attached). The past 8 gels all share the same trends: the bottom half of the gel fades out and is absent of any bands. I...

19 December 2020 3,619 1 View

What is the maximum passage number of SKOV-3 and SKOV-3-Luc cells?

Hello, for my experiment I'm working with SKOV-3-Luc cells, which are SKOV-3 cells stably/continuously expressing the firefly luciferase enzyme. These cells are obtained by the transfection of...

25 November 2020 3,219 3 View

Masters thesis subject - International humanitarian law/ International criminal law?

Hello I am a master's student and I'm searching for a current debate in the field of international humanitarian law or international criminal law. Is there somebody who can help me with this?...

08 November 2020 1,571 13 View

Is it possible that a IncX and IncF plasmid fused?

I recently performed plasmid extraction and nanopore sequencing. I have found what looks like a plasmid with replicon types IncX and IncF. Even with spAdes, canu, unicycler (illumina and nanopore...

29 September 2020 7,778 2 View

Can anyone tell me which species (or higher taxon) this is?

We found this small, maybe parasitic, crustacean in the North Sea offshore. The posterior body part is missing, but it looks quite characteristic. There is double-hooked rostrum, 2 antennae...

08 April 2020 6,842 9 View

Can I use time as a predictor variable in a panel regression?

Is it possible to generate a new variable "phase" that is based on the time the participants were questioned? I generated this new variable by dividing the time into three phases (beginning,...

30 March 2020 3,756 6 View

Dielectric constants of organic:aqueous mixtures, is linear addition a valid approximation?

I would like to compute dielectric constants of organic:aqueous mixtures. Would linear addition of the dielectric constants of the pure solvents give reliable results? For example, for a volume...

26 March 2020 5,939 3 View

Which SPSS test should I use?

For a small project, I have to calculate a few things via SPSS. However, I am not sure which tests to use. I have multiple variables. Independent variable: categorical variable with two levels....

12 March 2020 7,188 3 View

Is there a device which can dillute a gas-sample before it enters a continious-flow gas-analysator?

I want to measure gaseous NH3 emitted from slurry with the gas-analysator G2508 from Picarro. I expect the concentration to be ten and more times higher than the operating range of the...

02 October 2019 8,276 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

No title

01 March 2021 3,905 5 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

No module named 'tensorflow.keras.backend.tensorflow_backend' ?

System Description Cuda 11.2 GeForce GTX 1080ti tensorflow version 2.4.1 keras version 2.2.0 python version 3.8 I was getting the below error when I try to run my code on the...

25 February 2021 6,673 2 View