Using Paraformaldehyde (more than a week) for fixing the plant samples (flower buds) ?

11 November 2017 1 4K Report

By mistake I kept some for my flower bud samples in paraformaldehyde for more than a week (usual time if few hours), can i still wash them with 1x PBS and store them in 70% ethanol at 4 C ?

(Currently they are in 4% paraformaldehyde @ 4C from past 9 days)

Anyone went through the same problem ?

Dharmesh Jain

Thanks for the reply.

I tried using those samples, I work on pollen and samples were flower buds. After the paraformaldehyde treatment, I was unable to take out the pollen from the anthers.

When I tried applying more pressure, I ended up crushing all the pollen and bringing out other anther wall layer (Tapetum) out.

Any suggestion on that ?

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