Dharmesh Jain

5 Questions 9 Answers 0 Followers

Questions related from Dharmesh Jain

Heterozygous line showing no expression in qPCR ?

I ordered Arabidopsis mutants from ABRC and found that one of the mutant is hetrozygous (confirmed by genotyping), later when I performed qPCR, I did not see any amplification of that particular...

10 October 2018 6,661 0 View

How to check whether an overexpression line is homo or heterozygous, if the plant looses antibiotic resistance ?

I want to know whether my over-expression line is homo or heterozygous in genetic background. I know some methods but want to is there any way to find that. Methods I know- 1. Check segregation...

08 August 2018 1,988 0 View

Is it possible to detect Ion-leakage in Arabidopsis Anthers ?

Hello, I am looking for a protocol by which I can detect the Ion-leakage in the anthers of my Arabidopsis Mutants. I want to know the detailed protocol mentioning the tissue amount (anther...

06 June 2018 1,567 5 View

Using Paraformaldehyde (more than a week) for fixing the plant samples (flower buds) ?

By mistake I kept some for my flower bud samples in paraformaldehyde for more than a week (usual time if few hours), can i still wash them with 1x PBS and store them in 70% ethanol at 4 C...

11 November 2017 4,246 1 View

Why am I getting multiple bands on gel after PCR amplification?

I am performing PCR for my several isolates, but in one isolate I'm getting multiple bands after the amplification. What could the reason be? I'm using a single set of primers.

10 October 2013 731 36 View