Hello,
I am looking for a protocol by which I can detect the Ion-leakage in the anthers of my Arabidopsis Mutants.
I want to know the detailed protocol mentioning the tissue amount (anther numbers) and steps to be followed.
TIN
Cheers,
Dharmesh
If I get you right, you can use electrolyte conductivity meter. Check the publications below for details. I hope they help
Sørensen, A., Lauridsen, E. B., & Thomsen, K. (1996). Electrical conductivity test (Doctoral dissertation, Danida Forest Seed Centre).
Varghese, B., Naidoo, C., & Pammenter, N. W. (2016). The use of plant stress biomarkers in assessing the effects of desiccation in zygotic embryos from recalcitrant seeds: challenges and considerations. Plant Biology, 18(3), 433-444.
Hello Dharmesh,
Take 1gm of fresh anther into 50 mL falcon tubes with 25 mL of 0.4 M mannitol solution and mix under agitation for 1 h. Measure the electrical conductivity of the solution at room temperature using a conductivity meter. The total conductivity (CT) of the solution could be determined either leaving the sample overnight at -30oC or, autoclave. Electrolyte leakage (L) is calculated using below mentioned eq :
L = (C(1h) / CT )*100
Best wishes.
Deepak
Dear all,
Thanks for the replies.
I will try to follow the suggestion.
Can anyone of you please suggest me some reference in which authors used anther samples.
As they are small and not easy to handle.
Thanks,
Dear Dharmesh, have a look to the attached file!
Available on: http://www.pnas.org/content/106/13/5412
Regards
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