Using in-fusion cloning connects two segments, the vector fragment 10693bp, the PCR-amplified fragment 1668bp, how much of the molar ratio ?

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What exactly is RAG-LLM doing? Isn’t it data engineering?

What exactly is Retrieval Augmented Generation for Large Language Model doing? Isn’t it data engineering?

30 July 2024 7,376 3 View

After a lot of feature engineering for CTR modeling, it feels like it's basically the end of iteration? I mean, it's not cost-effective to keep doing?

After a lot of feature engineering for click-through rate modeling, it feels like it's basically the end of iteration? I mean, it's not cost-effective to keep doing it?

29 July 2024 4,955 0 View

All math can be explained by iterator of code?

all math can be traversed by code? all math can be translate to code?

26 July 2024 9,530 0 View

Abaqus不收敛，警告里面出现下列问题，需要更改哪个地方？?

The system matrix has 172 negative eigenvalues. Displacement increment for contact is too big. Displacement increment for contact is too big. The strain increment has exceeded fifty times the...

05 July 2024 3,839 2 View

What is the effect for the CTR model, adding the tag-id with the highest number of user clicks/purchases on the item's tag as user-side features?

22 June 2024 3,588 1 View

We are not able to successully reproduce 3t3-L1 cell differentiation, what could be the reasons?

Greetings all, My group has been trying to reproduce 3t3-L1 cell (preadipocytes) differentiation. The typical protocol involves 3 steps: the first step and second step both last for 2 days, and...

18 June 2024 2,728 1 View

How to Construct Synthetic Phage Library?

Hi, I am trying to make synthetic phage library. Right now , do people still use Kunkel method or other methods? Since it looks like Kunkel method is an old method. I am looking for more...

09 June 2024 6,780 1 View

The primary problem that LLMs solved is small sample learning, right?

The primary problem that large language models solved is small sample learning, right?

03 June 2024 3,305 2 View

based on GPT-3, Text generation really solves all tasks, or solves only text generation?

based on GPT-3

01 June 2024 5,445 3 View

ChatGPTs can really replace search engine?

Or they are only complement to each other

01 June 2024 6,021 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View